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Rapid high-performance liquid chromatographic assay of dorzolamide in rabbit aqueous humor

โœ Scribed by Adriana Maltese; Claudio Bucolo

Publisher
John Wiley and Sons
Year
2002
Tongue
English
Weight
59 KB
Volume
16
Category
Article
ISSN
0269-3879

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โœฆ Synopsis

A rapid high-performance liquid chromatographic method using a C 18 reversed-phase column (Hypersil ODS) with UV detection at 254 nm and a simple pre-treatment of samples is presented for the analysis of dorzolamide, a carbonic anhydrase inhibitor, in rabbit aqueous humor. A water solution containing 2% ZnSO 4 ร7H 2 O was used to deproteinize aqueous humor samples. The mobile phase consisted of 7% CH 3 CN and 93% of a solution containing 1% TEA adjusted to pH = 3.5 with H 3 PO 4 . Paracetamol was found to be a suitable internal standard. The standard curves were linear in the detection range. The precision and the accuracy were <5% for both intra-and inter-day assays.

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