Rapid and Specific Precolumn Extraction High-Performance Liquid Chromatographic Assay for Bupivacaine in Human Serum

✍ Scribed by Samy Emara; Alaa Khedr; Hassan Askal

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 355 KB
• Volume: 10
• Category: Article
• ISSN: 0269-3879
• DOI: 10.1002/(sici)1099-0801(199605)10:3<131::aid-bmc572>3.0.co;2-w

✦ Synopsis

A rapid and specific HPLC assay for quantitative determination of bupivacaine in human serum is described.

The technique incorporates an on-line sample clean-up system followed by reversed-phase chromatography with UV detection. The proposed method uses a column-switching technique and protein-coated Lichrosorb Rp-8 as a precolumn together with Lichrosorb RP-18 as an analytical column. The total run time for an injection of serum sample was 10min. This procedure offers a sensitive assay without the need for time-consuming extractions. The average bupivacaine recoveries over a concentration range of 150-600 nglmL ranged from 99.12 to 101.02%, and relative standard deviations ranged,from 1.15 to 1.78%.