High performance liquid chromatographic determination of levomepromazine in human breast milk and serum using solid phase extraction

✍ Scribed by Tadashi Ohkubo; Ritsuko Shimoyama; Kazunobu Sugawara

• Publisher: John Wiley and Sons
• Year: 1993
• Tongue: English
• Weight: 205 KB
• Volume: 7
• Category: Article
• ISSN: 0269-3879
• DOI: 10.1002/bmc.1130070411

✦ Synopsis

A high performance liquid chromatographic (HPLC) method has been developed for the determination of levomepromazine in human breast milk and serum. The levomepromazine was extracted by a rapid and simple extraction method using a Sep-Pak CI8 cartridge. The extracts were separated by HPLC on a C8 bonded reversed phase column and detected by UV absorbance at 254 nm. There was no interference with endogenous substance in human breast milk and serum. A linear relationship was obtained for the levomepromazine over the concentration range of 10-300 ng/mL. The recoveries of levomepromazine added to human breast milk and serum were 92.5-99.1% and 86.9-103.9'/0, respectively. ODS stationary phase columns (Murakami et al., 1982;