Determination of phenobarbital in plasma by micellar liquid chromatography

✍ Scribed by Y. Martín-Biosca; S. Sagrado; R. M. Villanueva-Camãnas; M. J. Medina-Hernández

• Publisher: John Wiley and Sons
• Year: 2000
• Tongue: English
• Weight: 59 KB
• Volume: 14
• Category: Article
• ISSN: 0269-3879
• DOI: 10.1002/(sici)1099-0801(200004)14:2<113::aid-bmc935>3.0.co;2-5

✦ Synopsis

A new liquid chromatographic procedure for the determination of phenobarbital in plasma samples is described. The proposed system uses a Spherisorb octadecyl-silane ODS-2 C 18 analytical column, a guard column of similar characteristics, and a 0.03 M CTAB-3% 1-propanol at pH 7 mobile phase. The UV detector was set at 250 nm. Butabarbital was used as internal standard. Sample preparation only required the addition to the plasma samples of a 0.1 M SDS solution at pH 3 and centrifugation before injection into the chromatographic system. The limit of detection was 0.83 mg/mL of phenobarbital in plasma samples. The coefficients of variation were lower than 7.5%.