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Direct Assay of Tinidazole in Human Serum by Micellar Liquid Chromatography

✍ Scribed by D. Habel; S. Guermouche; M. H. Guermouche

Publisher: John Wiley and Sons
Year: 1997
Tongue: English
Weight: 144 KB
Volume: 11
Category: Article
ISSN: 0269-3879
DOI: 10.1002/(sici)1099-0801(199701)11:1<16::aid-bmc619>3.0.co;2-k

No coin nor oath required. For personal study only.

✦ Synopsis

A liquid chromatographic procedure for the direct determination of tinidazole in human serum is presented. It includes the use of a micellar mobile phase consisting of SDS (5.10-' M): propan-1-01; (94:6; vlv) and a pBondapak CN column with W detection at 320 nm. No solvent extraction or deproteinization are necessary. The linearity (0.1-10 mg L), the precision (3%), the reproducibility (1.3%), the recovery (99%), and the detection limit (0.1 mg L) in the tinidazole determination are comparable and sometimes greater than the corresponding tinidazole parameters when deproteinization and conventional reversed-phase HPLC are used. One hundred injections of serum samples do not affect the column life. The procedure is applied to ascertain the pharmacokinetics of 10 mglkg of tinidazole.

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