Simultaneous determination of creatine, uric ccid, creatinine and hippuric acid in urine by high performance liquid chromatography

✍ Scribed by Yang Yue-dong

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 49 KB
• Volume: 12
• Category: Article
• ISSN: 0269-3879
• DOI: 10.1002/(sici)1099-0801(199803/04)12:2<47::aid-bmc717>3.0.co;2-y

✦ Synopsis

A simple method for the simultaneous determination of creatine, uric acid, creatinine and hippuric acid in urine using reversed-phase high performance liquid chromatography was described. Chromatography was performed on a Nova-Pak C 18 (3.9؋ 150 mm) column with a mobile phase of 0.02 mol/L KH 2 PO 4 , and UV detection at 220 nm. The recoveries of creatine, uric acid, creatinine and hippuric acid were 103.13, 99.86, 98.26 and 98.24%, respectively. The calibration curves were linear within the concentration range of 2-200 g/mL for creatine, uric acid and hippuric acid, and 2-400 g/mL for creatinine. The detection limits of the method were 0.69, 0.21, 0.10 and 0.095 g/mL for creatine, uric acid, creatinine and hippuric acid, respectively. This method has been applied to the analysis of urine samples from normal subjects and some patients.