Simultaneous determination of ascorbic acid and dehydroascorbic acid by HPLC with postcolumn derivatisation and fluorometric detection
β Scribed by Michaela Capellmann; Hermann M. Bolt
- Book ID
- 112291211
- Publisher
- Springer
- Year
- 1992
- Tongue
- English
- Weight
- 407 KB
- Volume
- 342
- Category
- Article
- ISSN
- 1618-2650
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π SIMILAR VOLUMES
New methods are presented for measuring total vitamin C and the ascorbic acid/dehydroascorbic acid ratio in gastric juice. Extracts are prepared from a gastric juice which are suitable for direct injection onto a Waters Nova-pak C , , Radial-pak cartridge for high performance liquid chromatography (
## Abstract Reliable measurement of the reduced and oxidized forms of ascorbic acid (AA) is challenging because they are highly reactive and unstable compounds. Detection of small amounts of AA and dehydroascorbic acid (DHAA) is essential for determining the biochemical function of the vitamin. Whi
The adaptation of an isocratic reversed-phase ion-pairing high performance liquid chromatographic method in combination with derivatization of dehydroascorbic acid by l&phenylenediamine prior to injection for simultaneous determination of ascorbic acid and dehydroascorbic acid in plant materials is