The polyamines putrescine, cadaverine, spermidine, and spermine and the corresponding mono-N-acetylpolyamines can be separated as their dimethylaminoazobenzenesulfonyl derivatives in a single analysis in less than 22 min. The method employs reversed-phase high-performance liquid chromatography (Sphe
Simultaneous determination of amino acids and biogenic amines by reversed-phase high-performance liquid chromatography of the dabsyl derivatives
✍ Scribed by Ingolf Krause; Annette Bockhardt; Herbert Neckermann; Thomas Henle; Henning Klostermeyer
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 793 KB
- Volume
- 715
- Category
- Article
- ISSN
- 1873-3778
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All 24 dansyl amino acids were separated by reverse-phase high-performance liquid chromatography on Develosil C8-5, using a linear gradient made from Tris-HCl buffer (pH 7.75) and methanol. A linear relationship between the amount of sample and peak area was found over the range of 6 to 300 ng (0.02
## Abstract Highly hydrophilic compounds belonging to biogenic amines were analysed in the reversed‐phase system, modified with the addition of ionic liquids: 1‐ethyl‐3‐methyl‐imidazolium hexafluorophosphate (EMIM PF~6~) and chaotropic salt NaPF~6~ on Discovery HS C18 column at acidic conditions. T