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Analysis of polyamines as their dabsyl derivatives by reversed-phase high-performance liquid chromatography

✍ Scribed by Pertti Koski; Ilkka M. Helander; Matti Sarvas; Martti Vaara

Publisher: Elsevier Science
Year: 1987
Tongue: English
Weight: 439 KB
Volume: 164
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(87)90395-2

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✦ Synopsis

The polyamines putrescine, cadaverine, spermidine, and spermine and the corresponding mono-N-acetylpolyamines can be separated as their dimethylaminoazobenzenesulfonyl derivatives in a single analysis in less than 22 min. The method employs reversed-phase high-performance liquid chromatography (Spherisorb S5 ODS2 column) with an acetonitrile/acetate buffer gradient elution system and detection in the visible (436 nm) region. The detection limit for a single dimethylaminoazobenzenesulfonylpolyamine is less than 2 pmol.

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