𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Analysis of dansyl amino acids by reverse-phase high-performance liquid chromatography

✍ Scribed by Norio Kaneda; Michihiko Sato; Kunio Yagi

Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
394 KB
Volume
127
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

✦ Synopsis

All 24 dansyl amino acids were separated by reverse-phase high-performance liquid chromatography on Develosil C8-5, using a linear gradient made from Tris-HCl buffer (pH 7.75) and methanol. A linear relationship between the amount of sample and peak area was found over the range of 6 to 300 ng (0.02-I nmol) of dansyl derivatives. An application of this method to the NH*-terminal analysis of lysozyme is described.

πŸ“œ SIMILAR VOLUMES

Analysis of eleven capsaicinoids by reve
Analysis of eleven capsaicinoids by reversed-phase high performance liquid chromatography
✍ Marie-NoΓ«lle Maillard; Pierre Giampaoli; Hubert M. J. Richard πŸ“‚ Article πŸ“… 1997 πŸ› John Wiley and Sons 🌐 English βš– 129 KB πŸ‘ 2 views

More than 90 dierent capsicums are found all over the world. They contain capsaicinoids, which are responsible for the hot and pungent Β―avour. After extraction by a methanolΒ±water mixture (60 : 40, v/v) from capsicum oleoresins, 13 compounds were separated by analytical HPLC on a C18 Hypersil column

Desalting of nucleotides by reverse-phas
Desalting of nucleotides by reverse-phase high-performance liquid chromatography
✍ Dexter B. Northrop; Colin J. Newton πŸ“‚ Article πŸ“… 1980 πŸ› Elsevier Science 🌐 English βš– 165 KB

Chromatography of AMP, NAD+, or NADH on a reverse-phase C,, Porasil B column rapidly removes ammonium formate or potassium phosphate from 90% of the nucleotide. Earlier reports showed these salts could not be separated from nucleotides by conventional desalting using gel filtration.