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Desalting of nucleotides by reverse-phase high-performance liquid chromatography

✍ Scribed by Dexter B. Northrop; Colin J. Newton

Publisher: Elsevier Science
Year: 1980
Tongue: English
Weight: 165 KB
Volume: 103
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(80)90623-5

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✦ Synopsis

Chromatography of AMP, NAD+, or NADH on a reverse-phase C,, Porasil B column rapidly removes ammonium formate or potassium phosphate from 90% of the nucleotide. Earlier reports showed these salts could not be separated from nucleotides by conventional desalting using gel filtration.

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