Desalting and concentration of proteins in dilute solution using reversed-phase high-performance liquid chromatography

✍ Scribed by Thomas Pohl; Roza Maria Kamp

• Publisher: Elsevier Science
• Year: 1987
• Tongue: English
• Weight: 296 KB
• Volume: 160
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(87)90065-0

✦ Synopsis

A rapid method for desalting and concentrating dilute protein solutions using short reversed-phase columns (3-4 cm) has been described. The recovery of proteins is usually 90-100%. The method is simple and rapid and allows the desalting and concentration of protein samples simultaneously. A wide variety of proteins in the range up to 80 kDa can be desalted in microgram to milligram amounts, and volumes up to 1 liter can be concentrated to a few milliliters by a single injection.