✦ LIBER ✦

Simulation of protein crystal nucleation

✍ Scribed by Matteo Pellegrini; Stephanie W. Wukovitz; Todd O. Yeates

Publisher: John Wiley and Sons
Year: 1997
Tongue: English
Weight: 132 KB
Volume: 28
Category: Article
ISSN: 0887-3585
DOI: 10.1002/(sici)1097-0134(199708)28:4<515::aid-prot5>3.0.co;2-8

No coin nor oath required. For personal study only.

✦ Synopsis

To attempt to understand the physical principles underlying protein crystallization, an algorithm is described for simulating the crystal nucleation event computationally. The validity of the approach is supported by its ability to reproduce closely the wellknown preference of proteins for particular space group symmetries. The success of the algorithm supports a recent argument that protein crystallization is limited primarily by the entropic effects of geometric restrictions imposed during nucleation, rather than particular energetic factors. These simulations provide a new tool for attacking the problem of protein crystallization by allowing quantitative evaluation of new ideas such as the use of racemic protein mixtures.

📜 SIMILAR VOLUMES

Protein crystal nucleation: Recent notio

Protein crystal nucleation: Recent notions

✍ Christo N. Nanev 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 537 KB

Probabilistic approach to protein crysta

Probabilistic approach to protein crystal nucleation

✍ Christo N. Nanev; Ivaylo Dimitrov 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 185 KB 👁 1 views

## Abstract Simple probabilistic model for step‐wise growth of polymers is used for making some parallels with the nucleation of protein crystals. Although the considerations are made within 1D case, this approximation still shows some important peculiarities of protein crystal nucleation and growt

Application of mean-separation-works met

Application of mean-separation-works method to protein crystal nucleation

✍ Christo N. Nanev 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 142 KB 👁 1 views

## Abstract Using __mean‐separation‐works__ method of Stranski and Kaischew calculations of nucleus form and energy barrier for its formation are performed for globular protein crystals. This is done on the basis of a simple model suggested for crystal nucleation of such proteins. The prerequisite

Protein nucleation and crystallization b

Protein nucleation and crystallization by homologous protein thin film template

✍ Eugenia Pechkova; Claudio Nicolini 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 244 KB

## Abstract A new method of protein nucleation and crystallization based on Langmuir–Blodgett technology is here utilized for the template stimulation of crystal growth of so far non‐crystallized proteins. Microcrystals (60–120 μm) of bovine cytochrome P450scc and human protein kinase CKII alpha su

Molecular Simulations of Methane Hydrate

Molecular Simulations of Methane Hydrate Nucleation

✍ John A. Ripmeester; Saman Alavi 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 English ⚖ 121 KB 👁 1 views

On slow protein crystal nucleation: clus

On slow protein crystal nucleation: cluster-cluster aggregation on diffusional encounters

✍ C. N. Nanev 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 182 KB 👁 1 views

## Abstract With a view to experimental results on protein crystal nucleation the effects of cluster coalescence are probed semi‐quantitatively. The steric association restriction, which stems from the patchy surface of the protein molecules, explains both experimentally measured low crystal nuclea