## Abstract A method for drying polyacrylamide slab gels of various size and gel composition (T 7.5β15 %, C 2.67 %) is described. The destained gels, containing glycerol, are overlayered with gelatin and dried. Transparent and flexible sheets are obtained which can be stored for extended periods an
Simple method for quantitive densitometry of polyacrylamide gels using fast green
β Scribed by Martin A. Gorovsky; Kathryn Carlson; Joel L. Rosenbaum
- Publisher
- Elsevier Science
- Year
- 1970
- Tongue
- English
- Weight
- 637 KB
- Volume
- 35
- Category
- Article
- ISSN
- 0003-2697
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π SIMILAR VOLUMES
A simple method for protein recovery from gel slices following polyacrylamide gel electrophoresis is proposed which requires neither additional devices nor much skill. The method is based on reversed electrophoresis using a discontinuous conductivity gradient which is in turn maintained by a gradien
dling and storage. The overall quality ofthe second-dimension gel is improved by eliminating the dark carrier ampholyte streak at the front of the basic end of the gel as well as the SDS-Triton artifact at the acidic end. The degree of protein resolution is essentially identical for the two techniqu