We are presenting a simple method for the quantitation of the radioactivity contained in individual bands of polyacrylamide or agarose gels following electrophoresis. Our technique essentially consists of localizing the individual bands by staining or another appropriate method, cutting out the indi
A simple method for measuring radioactivity in autoradiograms of polyacrylamide gels
โ Scribed by Jerry W. Smith; Joseph C. Glorioso
- Publisher
- Elsevier Science
- Year
- 1977
- Weight
- 586 KB
- Volume
- 28
- Category
- Article
- ISSN
- 0020-708X
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Electrophoresis in polyacrylamide gels is widely used for analysis and characterization of proteins and mixtures of proteins. It is frequently necessary to obtain estimates of the radioactivity of protein bands in the gels, and of the relative amounts of two radionuclides, generally 3H ,and l\*C. Ca
dling and storage. The overall quality ofthe second-dimension gel is improved by eliminating the dark carrier ampholyte streak at the front of the basic end of the gel as well as the SDS-Triton artifact at the acidic end. The degree of protein resolution is essentially identical for the two techniqu