A simple generally applicable method for measuring radioactive substances separated by polyacrylamide and agarose-gel electrophoresis

✍ Scribed by Ernest Albanese; David Goodman

• Publisher: Elsevier Science
• Year: 1977
• Tongue: English
• Weight: 598 KB
• Volume: 80
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(77)90625-x

✦ Synopsis

We are presenting a simple method for the quantitation of the radioactivity contained in individual bands of polyacrylamide or agarose gels following electrophoresis. Our technique essentially consists of localizing the individual bands by staining or another appropriate method, cutting out the individual bands from the dried gel, and dissolving them in H202/NH40H for scintillation counting. This method has been used for various isotopes, for double-label experiments, and for various types of gel. Our method permits the quantitative determination of radioactivity by counting only the individual radioactive bands, thus avoiding the need to obtain and count a very large number of small slices or to suffer the inevitable loss of resolving power.