dling and storage. The overall quality ofthe second-dimension gel is improved by eliminating the dark carrier ampholyte streak at the front of the basic end of the gel as well as the SDS-Triton artifact at the acidic end. The degree of protein resolution is essentially identical for the two techniqu
A simple method for drying polyacrylamide slab gels using glycerol and gelatin
β Scribed by Octavian Popescu
- Publisher
- John Wiley and Sons
- Year
- 1983
- Tongue
- English
- Weight
- 253 KB
- Volume
- 4
- Category
- Article
- ISSN
- 0173-0835
No coin nor oath required. For personal study only.
β¦ Synopsis
Abstract
A method for drying polyacrylamide slab gels of various size and gel composition (T 7.5β15 %, C 2.67 %) is described. The destained gels, containing glycerol, are overlayered with gelatin and dried. Transparent and flexible sheets are obtained which can be stored for extended periods and may also be used for autoradiography.
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