The mutual interference between the second-derivative bands of tyrosine and tryptophan in proteins has been evaluated in terms of the ratio r between two peak-to-peak distances. The r values have been found to be well related, although not linearly, to the tyrosine/ tryptophan ratio in both model co
Simple determination of nitrate in soils by second-derivative spectroscopy
โ Scribed by A. SEMPERE; J. OLIVER; C. RAMOS
- Book ID
- 108718006
- Publisher
- John Wiley and Sons
- Year
- 1993
- Tongue
- English
- Weight
- 447 KB
- Volume
- 44
- Category
- Article
- ISSN
- 1351-0754
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Dipicolinic acid was extracted from approximately 0.1 mg spores or 0.5 ml of sporulating culture with 20 mM HCl for 10 min at 100 degrees C. The suspension was diluted with 5 mM Ca2+, 100 mM Tris, pH 7.6, centrifuged, and the first derivative of the uv absorbance spectrum recorded from 275 nm to 285