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Simultaneous determination of tyrosine and tryptophan residues in proteins by second-derivative spectroscopy

✍ Scribed by Luigi Servillo; Giovanni Colonna; Ciro Balestrieri; Raffaele Ragone; Gaetano Irace

Publisher: Elsevier Science
Year: 1982
Tongue: English
Weight: 449 KB
Volume: 126
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(82)90512-7

No coin nor oath required. For personal study only.

✦ Synopsis

The mutual interference between the second-derivative bands of tyrosine and tryptophan in proteins has been evaluated in terms of the ratio r between two peak-to-peak distances. The r values have been found to be well related, although not linearly, to the tyrosine/ tryptophan ratio in both model compound mixtures and proteins. A method for the simultaneous determination of the two major protein chromophores at neutral pH has been developed.

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