Simultaneous determination of otilonium bromide and diazepam by first-derivative spectroscopy

✍ Scribed by Carlo Mannucci; Jacopo Bertini; Aldo Cocchini; Andrea Perico; Franco Salvagnini; Antonio Triolo

• Publisher: John Wiley and Sons
• Year: 1992
• Tongue: English
• Weight: 287 KB
• Volume: 81
• Category: Article
• ISSN: 0022-3549
• DOI: 10.1002/jps.2600811209

✦ Synopsis

A rapid, simple assay procedure was developed for simultaneous analysis of otilonium bromide, a smooth-muscle relaxant, and diazepam in tablets containing 20 mg of otilonium bromide and 2 mg of diazepam (20:2 tablets) or 40 mg of otilonium bromide and 2 mg of diazepam (40:2 tablets) by "zero-crossing" first-derivative spectroscopy. The tablets were dissolved in 0.01 N HCl, mixtures were centrifuged at 3500 rpm (2472 x g) for 5 min, and first-derivative spectra were recorded. The absolute values of the derivative were measured at 264 nm for determination of otilonium bromide and between 406 and 408 nm (380 nm for analysis of 40:2 tablets) for determination of diazepam. The method is linear, quantitative, and reproducible and can also be used for the tablet dissolution test. Ten tablets of the same batch were analyzed by the described method and by a high-performance liquid chromatographic method, and the results were in good agreement.