Simultaneous determination of naproxen and its desmethyl metabolite in human serum by second-derivative synchronous fluorescence spectrometry

✍ Scribed by Dimitrios G. Konstantianos; Pinelopi C. Ioannou; Efstratios Stratikos

• Publisher: Elsevier Science
• Year: 1994
• Tongue: English
• Weight: 493 KB
• Volume: 290
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(94)80037-5

✦ Synopsis

A simple, rapid, sensitive and selective method for the simultaneous determination of the non-stetoidal anti-inflammatory drug naptoxen (NAP) and its main metabolite the 6-demethylated derivative (DNAP) in human serum is described. The method is based on the intrinsic fluorescence of both acids in alkaline aqueous solution. The broad-band overlapping conventional spectra of both compounds are resolved by means of second-derivative synchronous fluorescence specttometry, thus obviating the need for pre-analysis sample separation techniques. NAF' and DNAP ate determined in serum supetnatant solution after removal of proteins with acetonittile at pH 11.7. Recoveries from seta spiked with NAP (S-70 pg ml-') and DNAP (0.25-5 gg ml-') ranged from 95 to 106% (mean 99.6%) and from 95 to 104% (mean lOO.l%o), respectively.