𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Silicone-based microcarriers: preparation and BHK cell culture

✍ Scribed by Emir B. Denkbaş; Allan S. Hoffman; Erhan Pişkin

Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
664 KB
Volume
58
Category
Article
ISSN
0923-0467

No coin nor oath required. For personal study only.

✦ Synopsis

Spherical polymeric microbeads were produced by suspension cross-linking of hydroxyl-terminated polydimethylsiloxane prepolymers. Dimethylsiloxane-ethyleneoxide copolymer, dibutyltin dilaurate and methyltriethoxysilane were used as surfactant, catalyst and cross-linker, respectively. The surfaces of the microbeads were further modified by exposure of 3-aminopropyltriethoxysilane plasma in a glow-discharge apparatus. These silicone-based microbeads were used as microcarriers in culturing of baby hamster kidney (BHK) cells both in stationary and in submerged culture conditions. High attachment and growth were achieved on the glow-discharge treated silicone microbeads with moderate surface wettabilities (contact angle: 43-46").

📜 SIMILAR VOLUMES

Preparation and culture of hepatocyte on
Preparation and culture of hepatocyte on gelatin microcarriers
✍ Tao, Xu ;Shaolin, Li ;Yaoting, Yu 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 359 KB

## Abstract Porous gelatin microcarriers having a diameter of 80–100 μm were prepared by the suspension method using toluene as the oil phase. Rat hepatocytes were cultured on gelatin and cytodexIII microcarriers. The cells retained its spherical shape, which is similar __in vivo__, and showed no m

Microcarrier culture: Applications in bi
Microcarrier culture: Applications in biological production and cell biology
✍ Charles L. Crespi; Toshiko Imamura; Phaik-Mooi Leong; Robert J. Fleischaker; Hen 📂 Article 📅 1981 🏛 John Wiley and Sons 🌐 English ⚖ 742 KB

## Abstract This report reviews recent progress in micro carrier technology at the Massachusetts Institute of Technology. This progress includes new understanding of the nutritional needs of high‐cell density micro carrier cultures, the demonstration of cell growth on microcarriers in a hormone‐sup

The adaptation of bhk cells to a non-amm
The adaptation of bhk cells to a non-ammoniagenic glutamate-based culture medium
✍ A. Christie; M. Butler 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 241 KB 👁 1 views

Although glutamine is a major carbon source for mammalian cells in culture, its chemical decomposition or cellular metabolism leads to an undesirable excess of ammonia. This limits the shelflife of glutaminesupplemented media and may reduce the cell yield under certain conditions. We have attempted