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Preparation and culture of hepatocyte on gelatin microcarriers

โœ Scribed by Tao, Xu ;Shaolin, Li ;Yaoting, Yu

Publisher
John Wiley and Sons
Year
2003
Tongue
English
Weight
359 KB
Volume
65A
Category
Article
ISSN
0021-9304

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โœฆ Synopsis

Abstract

Porous gelatin microcarriers having a diameter of 80โ€“100 ฮผm were prepared by the suspension method using toluene as the oil phase. Rat hepatocytes were cultured on gelatin and cytodexIII microcarriers. The cells retained its spherical shape, which is similar in vivo, and showed no morphological changes to the flat state. Hepatocyte aggregates on microcarriers maintained higher metabolic functions than monolayer cells. Pore size of microcarrier plays an important role in the attachment and metabolic function of cells in culture. Phaseโ€contrast micrograph and cell activity showed that hepatocytes cultures on gelatin microcarrier of <1 ฮผm pore size is better than that of 5โ€“10 ฮผm. ยฉ 2003 Wiley Periodicals, Inc. J Biomed Mater Res 65A: 306โ€“310, 2003

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