✦ LIBER ✦

Hypothermic preservation of hepatocytes on gelatin gels

✍ Scribed by Peter J. Evans

Publisher: Elsevier Science
Year: 1994
Tongue: English
Weight: 458 KB
Volume: 18
Category: Article
ISSN: 1065-6995
DOI: 10.1006/cbir.1994.1023

No coin nor oath required. For personal study only.

✦ Synopsis

ABSTRACT

Hypothermic preservation of hepatocytes on gelatin gels allows hepatocytes to be stored for at least 9 days. The procedure is easy, inexpensive and does not require specialised equipment. The cells retain their morphology and are released as separate spherical entities by dissolving the gelatin layer at 37°. The recovered cells have an intact plasma membrane as judged by lactate dehydrogenase activity, attachment efficiency and subsequent monolayer formation in culture. Functional tests show that the cells recover quickly from the storage conditions. Rates of protein synthesis are maintained over a 6 day period and remain at 62% of the initial level on day 9. The liver specific, hormonal induction of the enzyme tyrosine aminotransferase is apparent throughout the culture period. In addition a phenotypic marker enzyme, γ‐glutamyl transpeptidase, remained at basal levels.

📜 SIMILAR VOLUMES

Maintenance of cytochrome P-450 levels i

Maintenance of cytochrome P-450 levels in hepatocytes preserved on gelatin gels

✍ Peter J. Evans 📂 Article 📅 1995 🏛 Elsevier Science 🌐 English ⚖ 282 KB

## Abstract In culture, cytochrome P‐450 levels fall rapidly with the result that hepatocytes are either used quickly or maintained in modified systems which prejudice their subsequent behaviour. In this study the effect of hypothermic preservation of hepatocytes on gelatin gels on levels of cytoch

Hypothermic preservation of hepatocytes.

Hypothermic preservation of hepatocytes. III. Effects of resuspension media on viability after up to 7 days of storage

✍ Diane C. Marsh; Julie A. Hjelmhaug; Paul K. Vreugdenhil; Julie A. Kerr; Mark J. 📂 Article 📅 1991 🏛 John Wiley and Sons 🌐 English ⚖ 907 KB

Hepatocyte suspensions provide a rapid method to determine how hypothermic storage affects liver cell metabolism and viability. Using these studies, improved methods of hypothermic liver preservation for transplantation may be developed. In this study, rat hepatocytes were cold-stored for up to 7 da

Preparation and culture of hepatocyte on

Preparation and culture of hepatocyte on gelatin microcarriers

✍ Tao, Xu ;Shaolin, Li ;Yaoting, Yu 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 359 KB

## Abstract Porous gelatin microcarriers having a diameter of 80–100 μm were prepared by the suspension method using toluene as the oil phase. Rat hepatocytes were cultured on gelatin and cytodexIII microcarriers. The cells retained its spherical shape, which is similar __in vivo__, and showed no m

On the nature of junction zones in gelat

On the nature of junction zones in gelatin gels

✍ Yu. B. Mel'Nichenko; Yu. P. Gomza; V. V. Shilov; S. I. Osipov 📂 Article 📅 1991 🏛 John Wiley and Sons 🌐 English ⚖ 430 KB

Studies on the rigidity of gelatine gels

✍ Tar, Ildikó ;Wolfram, Ervin 📂 Article 📅 1979 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 257 KB

## Abstract Gelatine gel samples formed in contact with phases of various polarity such as glass, plexiglass (PMMA) and air have been studied by a Röth‐type micro‐penetrometer. The experimental results showed that the penetrability of the gels was significantly effected by the adjacent phase. Compa

Effects of phenolic compounds on gelatio

Effects of phenolic compounds on gelation behavior of gelatin gels

✍ Jin Wu; Shih-Chien Chiu; Eli M. Pearce; T. K. Kwei 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 167 KB 👁 3 views

The effects of phenolic additives on the gelation behavior of gelatin gels were investigated using thermomechanical analysis (TMA) for study of gel-melting temperature, dynamic mechanical analysis (DMA) for study of gel-storage modulus and gel-aging stability, viscometry for study of gelation time,