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Maintenance of cytochrome P-450 levels in hepatocytes preserved on gelatin gels

✍ Scribed by Peter J. Evans

Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
282 KB
Volume
19
Category
Article
ISSN
1065-6995

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✦ Synopsis

Abstract

In culture, cytochrome P‐450 levels fall rapidly with the result that hepatocytes are either used quickly or maintained in modified systems which prejudice their subsequent behaviour. In this study the effect of hypothermic preservation of hepatocytes on gelatin gels on levels of cytochrome P‐450 was investigated. In marked contrast to conventional cultures, hypothermic preservation (10Β°) maintained, over a 6‐day period, cytochrome P‐450 at levels similar to those of the more stable cytochrome b~5~. Cell storage on gelatin at 25Β° was associated with a conversion of cytochrome P‐450 to cytochrome P‐420. The procedure at 10Β° provides a valuable tool for toxicity testing, hepatocyte conservation and distribution.

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