Quantification of attached cells in tissue culture plates and on microcarriers
โ Scribed by Rogerio Margis; Radovan Borojevic
- Publisher
- Elsevier Science
- Year
- 1989
- Tongue
- English
- Weight
- 345 KB
- Volume
- 181
- Category
- Article
- ISSN
- 0003-2697
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The rates of cell attachment of the anchorage-dependent mammalian cell line Vero t o the gelatin-based macroporous microcarrier Cultispher-G were determined under various conditions. An optimal rate of attachment (0.98 x min-') occurred by an intermittent stirring regimen of 3 min stirring at 40 rpm
The attachment and growth of L-929 cells on films made of Bombyx movi silk proteins-fibroin and sericin and their mixtures-was studied by a cell culture method. Both cell attachment and growth were dependent on a minimum of around 90% sericin in the mixture. The results from electron micrography as
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