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Attachment and growth of cultured fibroblast cells on PVA/chitosan-blended hydrogels

✍ Scribed by Koyano, Tomoe ;Minoura, Norihiko ;Nagura, Masanobu ;Kobayashi, Ken-ichi

Publisher: John Wiley and Sons
Year: 1998
Tongue: English
Volume: 39
Category: Article
ISSN: 0021-9304
DOI: 10.1002/(sici)1097-4636(19980305)39:3<486::aid-jbm20>3.0.co;2-7

No coin nor oath required. For personal study only.

✦ Synopsis

Hydrogels were prepared from poly(vinyl alcohol) and chitosan in various blend ratios. The water contents of the hydrogels were in the range of 65 to 75 wt %. The attachment and growth of fibroblast cells (L-929) on the hydrogels were studied with a cell culture method. On the hydrogels with more than 15 wt % chitosan content, the attached cells were able not only to remain viable but also to proliferate. The relative cell attachment after incubation for 30 h increased with increasing chitosan content in the hydrogels. Cell attachment and growth on the hydrogel with 40 wt % chitosan content exceeded those on collagen, a widely-used mammalian cell culture substrate. The morphology of the cells attached onto the hydrogels with a lower chitosan content was spherical, but in hydrogels with more than 15 wt % chitosan content, the number of spindleshaped cells increased with increasing chitosan content.

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