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Attachment and growth of fibroblast cells on polypeptide derivatives

✍ Scribed by Kang, Inn-Kyu ;Ito, Yoshihiro ;Sisido, Masahiko ;Imanishi, Yukio

Publisher
John Wiley and Sons
Year
1989
Tongue
English
Weight
840 KB
Volume
23
Category
Article
ISSN
0021-9304

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✦ Synopsis

Attachment and growth of mouse fibroblast cells on polypeptide derivatives with different wettabilities were studied in the presence or absence of serum proteins. In the presence of serum, a peak level of cell attachment was observed for substrates with a contact angle of around 70". However, no relationship was found between cell attachment and water contact angle of the substrate in the absence of serum. Ca2+-dependent cell attachment was observed on hydrophobic polypeptides in the absence of serum proteins. This sug-gests that Ca2'-dependent membrane proteins function as mediators for cell attachment to hydrophobic polypeptides. In the presence of serum proteins, it was found that cell attachment is affected by the metabolism, Ca2+, and the cytoskeleton of the cell. Cell growth rate on hydrophilic polypeptide substrates was higher than that on intermediate or hydrophobic polypeptide substrates. This demonstrates that the strong interaction between cells and substrates will be unfavorable for a dewebbing process during mitosis.

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