✦ LIBER ✦

Attachment and growth of cultured fibroblast cells on silk protein matrices

✍ Scribed by Minoura, Norihiko ;Aiba, Sei-Ichi ;Gotoh, Yohko ;Tsukada, Masuhiro ;Imai, Yohji

Publisher: John Wiley and Sons
Year: 1995
Tongue: English
Weight: 697 KB
Volume: 29
Category: Article
ISSN: 0021-9304
DOI: 10.1002/jbm.820291008

No coin nor oath required. For personal study only.

✦ Synopsis

The attachment and growth of L-929 cells on films made of Bombyx movi silk proteins-fibroin and sericin and their mixtures-was studied by a cell culture method. Both cell attachment and growth were dependent on a minimum of around 90% sericin in the mixture. The results from electron micrography as well as from the DSC measurements supported the notion that the mixture of the two proteins fibroin and sericin has a phase-separated structure in the solid state. The observed minimum of sericin in the cell attachment and growth is thought to be a result of this phase-separated structure. Films of pure component pro-teins (Le., 100% fibroin or sericin) exhibited as high a cell attachment and growth as collagen, a widely used mammalian cell culture substrate. However, a morphological study of the attached cells revealed that the cells attached to silk fibroin were extended and had a spindle shape, just like the cells attached to collagen, while the cells attached to the silk sericin had a different shape. It is concluded, therefore, that the attachment condition on silk fibroin is ideal for the viability, growth and function of the cells.

📜 SIMILAR VOLUMES

Attachment and growth of cultured fibrob

Attachment and growth of cultured fibroblast cells on PVA/chitosan-blended hydrogels

✍ Koyano, Tomoe ;Minoura, Norihiko ;Nagura, Masanobu ;Kobayashi, Ken-ichi 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English

Hydrogels were prepared from poly(vinyl alcohol) and chitosan in various blend ratios. The water contents of the hydrogels were in the range of 65 to 75 wt %. The attachment and growth of fibroblast cells (L-929) on the hydrogels were studied with a cell culture method. On the hydrogels with more th

Attachment and growth of fibroblast cell

Attachment and growth of fibroblast cells on polypeptide derivatives

✍ Kang, Inn-Kyu ;Ito, Yoshihiro ;Sisido, Masahiko ;Imanishi, Yukio 📂 Article 📅 1989 🏛 John Wiley and Sons 🌐 English ⚖ 840 KB

Attachment and growth of mouse fibroblast cells on polypeptide derivatives with different wettabilities were studied in the presence or absence of serum proteins. In the presence of serum, a peak level of cell attachment was observed for substrates with a contact angle of around 70". However, no rel

Fibroblast cell attachment and growth on

Fibroblast cell attachment and growth on nanoengineered sculptured thin films

✍ M. C. Demirel; E. So; T. M. Ritty; S. H. Naidu; A. Lakhtakia 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 198 KB

## Abstract Nanoengineered parylene‐C sculptured thin films (STFs) are deposited on glass and silicon substrates using a direct one‐step growth technique. The deposited STFs support fibroblast cell attachment and proliferation __in vitro__, which is an early indication of biocompatibility and bioac

Effect of the chemical modification of t

Effect of the chemical modification of the arginyl residue inBombyx mori silk fibroin on the attachment and growth of fibroblast cells

✍ Gotoh, Yohko ;Tsukada, Masuhiro ;Minoura, Norihiko 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 536 KB

We prepared matrices of Bombyx mori silk fibroin (SF) with different degrees of modification of arginyl residues by reaction between 1,2-cyclohexanedione (CHD) and SF. Two kinds of SF, namely native SF (NSF), obtained from the silk gland of silkworm larvae, and regenerated SF (RSF), prepared from co

Attachment, morphology, and protein expr

Attachment, morphology, and protein expression of rat marrow stromal cells cultured on charged substrate surfaces

✍ Qiu, Q. ;Sayer, M. ;Kawaja, M. ;Shen, X. ;Davies, J. E. 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 736 KB

Although surface charge has been shown to affect the adhesion and morphology of a variety of cell types, the interactions of bone marrow stromal cells with charged surfaces still remain unclear. A novel electrical stimulation system was used to investigate the interactions between rat bone marrow st

Primary retinal and cortical glial cell

Primary retinal and cortical glial cell cultures: Effects of medium and serum on attachment and growth

✍ Dr. M. C. Trachtenberg; K. M. Hokanson 📂 Article 📅 1986 🏛 John Wiley and Sons 🌐 English ⚖ 604 KB

Glial cells of the retina are anatomically distinctive and are thought to contribute importantly to retinal electrophysiology. However, no adequate preparation exists for studying them in isolation, in vitro. This report provides guidelines for primary retinal glial cultures (RET) and compares basal