✦ LIBER ✦

Identification and quantification of actin isoforms in vertebrate cells and tissues

✍ Scribed by C. A. Otey; M. H. Kalnoski; J. C. Bulinski

Publisher: John Wiley and Sons
Year: 1987
Tongue: English
Weight: 808 KB
Volume: 34
Category: Article
ISSN: 0730-2312
DOI: 10.1002/jcb.240340205

No coin nor oath required. For personal study only.

✦ Synopsis

The cytoskeletal protein actin exists in vertebrates as six different isoforms, which are difficult to identify conclusively because of a high degree (> 90%) of overall sequence homology. We have used IEF immunoblotting in combination with a panel of isoform-specific and -selective antibodies to analyze the actin isoform composition of nine tissues from adult rat. In three nonmuscle tissues (lung, spleen, and testis), we detected a previously unreported isoform that we identified as smooth muscle a. The IEF immunoblot technique was also used to quantify the proportions of the isoforms expressed in these nine rat tissues.

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