Microcarrier culture: Applications in biological production and cell biology

✍ Scribed by Charles L. Crespi; Toshiko Imamura; Phaik-Mooi Leong; Robert J. Fleischaker; Henri Brunengraber; William G. Thilly; Donald J. Giard

• Publisher: John Wiley and Sons
• Year: 1981
• Tongue: English
• Weight: 742 KB
• Volume: 23
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/bit.260231204

✦ Synopsis

Abstract

This report reviews recent progress in micro carrier technology at the Massachusetts Institute of Technology. This progress includes new understanding of the nutritional needs of high‐cell density micro carrier cultures, the demonstration of cell growth on microcarriers in a hormone‐supplemented, serum‐free medium, and the continuous cell propagation of epithelial cell types in microcarrier culture by medium modification that permits efficient bead‐to bead transfer. Also, a technique for obtaining large quantities of mitotic cells by selective detachment from microcarriers is reported. Finally, recent progress in interferon production from human foreskin fibroblasts grown on microcarriers is outlined: Our improvements in the interferon “super induction” process have increased the interferon yield per cell fivefold to tenfold.