Separation of the intermediates of leucine catabolism by high-performance liquid chromatography
โ Scribed by Theodore Page; William L. Nyhan
- Book ID
- 118316542
- Publisher
- Elsevier Science
- Year
- 1985
- Tongue
- English
- Weight
- 424 KB
- Volume
- 34
- Category
- Article
- ISSN
- 0006-2944
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## Abstract A method is presented for separation of tryptic glycopeptidesโcontaining oligosaccharides of the Nโasparagineโlinked type. High performance liquid Chromatography (HPLC) of glycopeptides on a C18 reverseโphase system eluted with a gradient of 0%โ50% acetonitrile in 0.1 M NaPO~4~ pH 2.2 r
For studies on the coenzyme B,,-dependent enzyme, leucine-2,3-aminomutase, (3R)-and (3S)-&leucines were synthesized. The 1 0-camphorsulfonamide pnitrobenzyl esters could be resolved by normal-phase HPLC. A much better separation was obtained by reversed-phase HPLC of the diastereomeric derivatives o