Separation of chloramphenicol intermediates by high-performance liquid chromatography on micropak-NH2 columns
✍ Scribed by Gy. Vigh; J. Inczédy
- Book ID
- 108334778
- Publisher
- Elsevier Science
- Year
- 1976
- Tongue
- English
- Weight
- 574 KB
- Volume
- 129
- Category
- Article
- ISSN
- 1873-3778
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High-pressure cation-exchange chromatography was used to separate three of the by-product compounds formed in the chloramphenicol production process. Baseline separation can be achieved within 8.5 min employing a l-m column packed with Zipax SCX pellicular cation exchanger and 0.05 M sodium sulphate
The least stable thiophenes were 5-(but-3-ene-l-ynyl)-2,2'bithiophene and 5-(but-3-ene-l-ynyl)-5'-methyl-2,2'-bithiophene. This lower stability is attributed to the molecules' highly conjugated side chains, which interact more strongly with the W A radiation; these side chains are absent in 2,2',5',