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Separation by high-performance liquid chromatography of (3R)- and (3S)-β-leucine as diastereomeric derivatives

✍ Scribed by D.John Aberhart; Jacques-Alain Cotting; Horng-Jau Lin

Publisher
Elsevier Science
Year
1985
Tongue
English
Weight
317 KB
Volume
151
Category
Article
ISSN
0003-2697

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✦ Synopsis

For studies on the coenzyme B,,-dependent enzyme, leucine-2,3-aminomutase, (3R)-and (3S)-&leucines were synthesized. The 1 0-camphorsulfonamide pnitrobenzyl esters could be resolved by normal-phase HPLC. A much better separation was obtained by reversed-phase HPLC of the diastereomeric derivatives obtained by treatment of.-leucine with Marfey's reagent (N*-(5-fluoro-2,4dinitrophenyl)+alaninamide).

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Separation by high-performance liquid chromatography of α- and β-amino acids: Application to assays of lysine 2,3-aminomutase and leucine 2,3-aminomutase
✍ D.John Aberhart 📂 Article 📅 1988 🏛 Elsevier Science 🌐 English ⚖ 425 KB

Several pairs of alpha- and beta-amino acids were well separated as the ortho-phthalaldehyde derivatives by reversed phase HPLC. These included alpha- and beta-lysine, leucine, tyrosine, serine, aminoisobutyric acid, and beta-hydroxyvaline and its positional isomer. The separation was applicable to