Separation of phenylthiohydantoin-amino acids by highpressure liquid chromatography
β Scribed by Eric W. Matthews; Peter G.H. Byfield; Iain MacIntyre
- Book ID
- 103146758
- Publisher
- Elsevier Science
- Year
- 1975
- Tongue
- English
- Weight
- 310 KB
- Volume
- 110
- Category
- Article
- ISSN
- 1873-3778
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π SIMILAR VOLUMES
All common phenyhhiohydantoin amino acids derived from sequencing are identifiable in 6 to 9 min with an isocratic method based on buffered aqueous acetonitrile, an ODS stationary phase, and programmed flow which increases during the run. With constant flow the analysis requires 16 min. The effects
A high-performance liquid chromatographic analytical system is described for the separation, identification, and quantitation of phenylthiohydantoin (PTH) derivatives of all amino acids normally encountered in proteins. The system employs reverse-phase chromatography on a PBondapak phenylalkyl stati
separation of ethyl acetate-extractable phenylthiohydantoin-amino acids by high-pressure liquid chromatography with organic solvent gradients have been modified to facilitate sequence determination of proteins labeled with several radioactive amino acids at a time. ## Several laboratories have re
## Abstract Capillary electrochromatography (CEC) was employed as a rapid and highβefficiency method for the isocratic separation of all 20 important phenylthiohydantoin (PTH) amino acids, the end products of Edman degradation during __N__βterminal protein sequencing. For this purpose, 75Β ΞΌm ID fus