All common phenyhhiohydantoin amino acids derived from sequencing are identifiable in 6 to 9 min with an isocratic method based on buffered aqueous acetonitrile, an ODS stationary phase, and programmed flow which increases during the run. With constant flow the analysis requires 16 min. The effects
Separation of phenylthiohydantoin-amino acids by high-performance liquid chromatography and some applications in dansyl Edman sequence analysis
โ Scribed by Maurizio Simmaco; Donatella Barra; Francesco Bossa
- Book ID
- 108335245
- Publisher
- Elsevier Science
- Year
- 1985
- Tongue
- English
- Weight
- 346 KB
- Volume
- 349
- Category
- Article
- ISSN
- 1873-3778
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๐ SIMILAR VOLUMES
A high-performance liquid chromatographic analytical system is described for the separation, identification, and quantitation of phenylthiohydantoin (PTH) derivatives of all amino acids normally encountered in proteins. The system employs reverse-phase chromatography on a PBondapak phenylalkyl stati
All the organic soluble amino acid phenylthiohydantoins are eluted (but not totally resolved) in under 40 min on either a Zorbax ODS or Permaphase ETH column. The superior resolution of the Zorbax ODS column and the complimentary characteristic of the Permaphase ETH column allow the isocratic analys