On the analysis of phenylthiohydantoin amino acids by high performance liquid chromatography
โ Scribed by Sven Erik Godtfredsen; Ronald W. A. Oliver
- Book ID
- 105651233
- Publisher
- Springer-Verlag
- Year
- 1980
- Weight
- 752 KB
- Volume
- 45
- Category
- Article
- ISSN
- 0105-1938
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๐ SIMILAR VOLUMES
All the organic soluble amino acid phenylthiohydantoins are eluted (but not totally resolved) in under 40 min on either a Zorbax ODS or Permaphase ETH column. The superior resolution of the Zorbax ODS column and the complimentary characteristic of the Permaphase ETH column allow the isocratic analys
The phenylthiohydantoins (Pth) of the common amino acids can be resolved in a single analysis using a 25 x 0.46cm DuPont Zorbax cyanopropylsilane (CN) column developed with a gradient of methanol/acetonitrile (17:3) in sodium acetate buffer, pH 5.4. The Zorbax CN columns exhibit greater durability,
A high-performance liquid chromatographic analytical system is described for the separation, identification, and quantitation of phenylthiohydantoin (PTH) derivatives of all amino acids normally encountered in proteins. The system employs reverse-phase chromatography on a PBondapak phenylalkyl stati