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Separation of dolichylpyrophosphoryloligosaccharides by liquid chromatography

✍ Scribed by Gerald B. Wells; Salvatore J. Turco; Barbara A. Hanson; Robert L. Lester

Publisher
Elsevier Science
Year
1981
Tongue
English
Weight
737 KB
Volume
110
Category
Article
ISSN
0003-2697

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✦ Synopsis

Fourteen dolichylpyrophosphoryloligosaccharides, precursors of the asparagine-linked oligosaccharides of glycoproteins, have been separated by liquid chromatography on silica gel. The dolichylpyrophosphoryyl-N-acetylglucosamine and the dolichylpyrophosphoryl-(N-acetylglucosamine),-(mannose),(glucose) 2,3 thus resolved were shown to retain their activity as substrates in enzyme catalyzed reactions. The chromatography procedure for the first time makes available many of these single intermediates for further study.

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