Separation of phospholipid derivatives by microcolumn liquid chromatography

✍ Scribed by Atsuo Sakamoto; Milos Novotny

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 416 KB
• Volume: 8
• Category: Article
• ISSN: 1040-7685
• DOI: 10.1002/(sici)1520-667x(1996)8:6<397::aid-mcs4>3.0.co;2-x

✦ Synopsis

Packed, fused silica capillaries were employed, at high chromatographic resolution, to separate complex mixtures of phospholipids derived from plant and animal sources. Benzoylation of phosphatidylcholine derivatives and dansylation of phosphatidylethanolamines were used in conjunction with miniaturized spectroscopic detectors. Under the conditions of stepwise gradient elution, detection limits for both derivatives were on the order of picomoles.