Separation of dimethylphosphatidates of alkylacyl glycerophosphocholine and their molecular species by high-performance liquid chromatography

✍ Scribed by Yasuhito Nakagawa; Kazuyuki Fujishima; Keizo Waku

• Publisher: Elsevier Science
• Year: 1986
• Tongue: English
• Weight: 565 KB
• Volume: 157
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(86)90210-1

✦ Synopsis

A high-performance liquid chromatography (HPLC) method for separation of the alkylacyl and diacyl analogs of choline glycerophospholipids (CGP) of guinea pig polymorphonuclear leukocytes and their molecular species is described. CGP were hydrolyzed with phospholipase D and then methylated with diazomethane to convert them to dimethylphosphatidates. The dimethylphosphatidates were then separated into the alkylacyl and diacyl subclasses by HPLC on a silica gel column within 15 min. The alkylacyl and diacyl analogs were then separated into individual molecular species by reverse-phase HPLC. Dimethylphosphatidates were resolved into 15 separate peaks, and 1 l-16 different molecular species of alkylacyl and diacyl glycerophosphocholines were identified on gas-liquid chromatography. The present results indicate that the CGP of polymorphonuclear leukocytes are composed of 27 major molecular species. In the alkylacyl subclass, the most predominant species was the 16:0-18:2 species (32%),