Rapid separation of anionic oligosaccharide species by high performance liquid chromatography
โ Scribed by Jacques U. Baenziger; Marvin Natowicz
- Publisher
- Elsevier Science
- Year
- 1981
- Tongue
- English
- Weight
- 386 KB
- Volume
- 112
- Category
- Article
- ISSN
- 0003-2697
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๐ SIMILAR VOLUMES
A rapid procedure utilizing high-performance liquid chromatography was developed for the separation of homooligomers of sialic acid (N-acetylneuraminic acid). The method utilizes the anion exchanger Mono-Q HR 5/S and can resolve sialyl oligomers with degrees of polymerization (DP) from 2 to 20 in 25
All common phenyhhiohydantoin amino acids derived from sequencing are identifiable in 6 to 9 min with an isocratic method based on buffered aqueous acetonitrile, an ODS stationary phase, and programmed flow which increases during the run. With constant flow the analysis requires 16 min. The effects