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Separation and Identification of Plant Glycerolipid Molecular Species by Particle Beam High Performance Liquid Chromatography–Mass Spectrometry

✍ Scribed by Ralf Nilsson; Conny Liljenberg

Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
426 KB
Volume
7
Category
Article
ISSN
0958-0344

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✦ Synopsis

A high performance liquid chromatograph was connected to a quadrupole mass spectrometer through a particle beam interface in order to investigate the suitability of the interface for the analysis of plant glycerolipid molecular species. Separation was performed on a reversed phase column with a high carbon loading (20%), together with mobile phase systems containing little or no water for optimal interface performance. Positive chemical ionization mass spectra which allowed unambiguous determination of molecular species were obtained for triacylglycerols, diacylglycerols, digalactosyldiacylglycerols, phosphatidylcholine and phosphatidylethanotamine. Baseline separation for all species was achieved in the case of triacylglycerols and diacylglycerols. Polar lipids could not be baseline separated when the mass spectrometer was used as the detector due to a peak broadening effect in the ion source.

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