Separation and identification of higher fullerenes by high-performance liquid chromatography coupled with electrospray ionization mass spectrometry

✍ Scribed by Kiyokatsu Jinno; Yoichiro Sato; Hideo Nagashima; Kenji Itoh

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 245 KB
• Volume: 10
• Category: Article
• ISSN: 1040-7685
• DOI: 10.1002/(sici)1520-667x(1998)10:1<79::aid-mcs11>3.0.co;2-h

✦ Synopsis

The separation and identification of higher fullerenes extracted from carbon soot were studied using high-performance liquid chromatography coupled Ž . with electrospray ionization mass spectrometry LC-ESI-MS . The mixture of fullerenes extracted from carbon soot was first separated by a preparative-scale Ž . column packed with monomeric octadecylsilica ODS , which mainly controls the elution order of fullerenes by their relative molecular mass. Then five batches of the fraction which should contain higher fullerenes larger than C were collected 84 and subsequently concentrated, followed by analytical-scale separation with a polymeric ODS column, which can elute fullerenes according to their shape and structure. Therefore, this stationary phase can separate many isomers of higher fullerenes. By identification using LC-ESI-MS, the existence of several higher fullerenes up to C and their isomers were confirmed. Temperature effects in the 98 separation of fullerenes were also examined and the results indicated that separation of C and C isomers could be achieved at a column temperature lower than 82 84

15ЊC using a polymeric ODS column.