Separation of basic proteins and peptides by capillary electrophoresis using a cationic surfactant

✍ Scribed by Ding, Weiliang ;Frit, James S.

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 548 KB
• Volume: 20
• Category: Article
• ISSN: 0935-6304
• DOI: 10.1002/jhrc.1240201102

✦ Synopsis

Incorporation of a low concentration of cetyltrimethylammonium bromide (CTAB) in the running electrolyte is shown to dynamically coat the silica capillary and to reverse the direction of electroosmotic flow. The CTAB coating prevented interaction of proteins with the capillary surface and enabled sharp peaks to be obtained in the electropherograms. A systematic study of experimental parameters demonstrated the importance of selecting a suitable buffer electrolyte and an appropriate pH. Excellent separations were obtained for five proteins, three enkephalins, and six dipeptides with an efficiency of approximately 500,000 theoretical plates per meter. The method developed is very simple to perform and was found to give excellent reproducibility.