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High molecular weight polyarginine as a capillary coating for separation of cationic proteins by capillary electrophoresis

✍ Scribed by Rick W. Chiu; Juan C. Jimenez; Curtis A. Monnig

Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
770 KB
Volume
307
Category
Article
ISSN
0003-2670

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✦ Synopsis

Fused silica capillaries treated with dilute solutions of high molecular weight polyarginine (PA) provide capillary electrophoresis separation efficiencies of more than 2 million theoretical plates per meter and improved migration time reproducibility for the analysis of protein mixtures. The non-covalently bound PA coating was stable for several days and could be easily regenerated by exposing the capillary to a dilute solution of PA for several minutes. A comparison of PA and polybrene (PB) treated capillaries indicates that the PA capillary is capable of providing higher separation efficiencies, but that PB provides a more stable coating when used under acidic conditions and with high ionic strength buffers.

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