Effect of zwitterionic surfactants on the separation of proteins by capillary electrophoresis
β Scribed by Bu Yi Gong; Dr. John W. Ho
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 385 KB
- Volume
- 18
- Category
- Article
- ISSN
- 0173-0835
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## Abstract In CZE, the adsorption of the proteins on the capillary wall is a common problem. This paper describes the simple method of utilizing zwitterionic buffer additives to improve the separation of proteins in untreated fused silica capillaries at neutral pH. Three kinds of zwitterion are ev
The capillary electrophoresis (CE) running electrolyte composition was optimized for the separation of selected glycoproteins. A good separation of the ovalbumin (OV) and alpha-acid glycoprotein (AAG) isoforms was achieved in 20 mmol x L(-1) N-(2-hydroxyethyl)piperazine-2'-(2-ethanesulfonic acid) (H