Effect of barium tetraborate on the separation of tryptic digests of proteins by zone electrophoresis in uncoated capillaries
β Scribed by Martha Elena Mendieta; Paolo Antonioli; Pier Giorgio Righetti; Attilio Citterio; Stephanie Descroix; Roberto Sebastiano
- Publisher
- John Wiley and Sons
- Year
- 2006
- Tongue
- English
- Weight
- 307 KB
- Volume
- 27
- Category
- Article
- ISSN
- 0173-0835
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## Abstract Fused silica open tubular columns treated with Superox 0.6 and 4 for capillary electrophoresis were evaluated. Excellent separations of proteins were obtained. Column effeiciencies of over 700,000 theoretical plates were achieved for basic proteins. The relative standard deviations (RSD
## Abstract In CZE, the adsorption of the proteins on the capillary wall is a common problem. This paper describes the simple method of utilizing zwitterionic buffer additives to improve the separation of proteins in untreated fused silica capillaries at neutral pH. Three kinds of zwitterion are ev