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Separations of proteins and proteolytic digests of proteins by capillary electrophoresis on superox-coated open tubular columns

✍ Scribed by Zhongxi Zhao; Abdul Malik; Milton L. Lee

Publisher
John Wiley and Sons
Year
1992
Tongue
English
Weight
487 KB
Volume
4
Category
Article
ISSN
1040-7685

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✦ Synopsis

Abstract

Fused silica open tubular columns treated with Superox 0.6 and 4 for capillary electrophoresis were evaluated. Excellent separations of proteins were obtained. Column effeiciencies of over 700,000 theoretical plates were achieved for basic proteins. The relative standard deviations (RSDs) in migration times for these proteins were below 1%. Comparisons were made of the electropherograms of α‐, β‐, γ‐, and δ‐chymotrypsins. Three different protein digestion reagents were used to produce complex peptide mixtures for analysis: trypsin, chymotrypsin, and pepsin. Each reagent produced a unique mixture of peptides. Capillary electrophoresis was used to elute the peptides, yielding highly efficient and reproducible separations.

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