Use of zwitterionic buffer additives to improve the separation of proteins in capillary zone electrophoresis

✍ Scribed by Fang, Xiao-hong ;Zhu, Tao ;Sun, Vi-hang

• Publisher: John Wiley and Sons
• Year: 1994
• Tongue: English
• Weight: 320 KB
• Volume: 17
• Category: Article
• ISSN: 0935-6304
• DOI: 10.1002/jhrc.1240171103

✦ Synopsis

Abstract

In CZE, the adsorption of the proteins on the capillary wall is a common problem. This paper describes the simple method of utilizing zwitterionic buffer additives to improve the separation of proteins in untreated fused silica capillaries at neutral pH. Three kinds of zwitterion are evaluated in the separation of acidic, neutral, and basic proteins, including their effect on protein efficiency, mobility, separation, and resolution; the difference between the effects of the different additives are also highlighted. The method has proved to be a possible means of reducing protein adsorption, especially for basic proteins.