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Separation of bacterial luciferase from oxidoreductases by affinity chromatography

✍ Scribed by TenLin S. Tsai

Publisher: Elsevier Science
Year: 1985
Tongue: English
Weight: 374 KB
Volume: 151
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(85)90063-6

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✦ Synopsis

The NADH-dependent and NADPH-dependent oxidoreductase activities associated with bacterial luciferase in Vibrio (Beneckea) harveyi can simultaneously be removed from purified luciferase through a Blue Sepharose CL-6B column. The result achieved with this one-step affinity chromatography is similar to that obtained with two sequential "reverse-affinity" chromatographies.

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